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What is 2San Vitamin D Test The Vitamin D Rapid Test Cassette detects levels of 25-hydroxyvitamin D 25OHD in human blood to screen for vitamin D deficiency. Vitamin D deficiency can severely impact health by increasing the risk of osteoporosis, multiple sclerosis, cardiovascular disease, diabetes, depression, and infections. The test works through competitive binding25OHD in the blood competes with 25OHD antigens on the test line for a limited amount of anti-25 OH vitamin D antibodies. The intensity of the resulting test line determines the vitamin D level. What are the key features Easy to Use Accurate at Home Test kit Quick Results Whats included Test Cassette Alcohol Pad Capillary Dropper Lancet Buffer Package Insert Colour Card Test Pack Usage How to use 2San Vitamin D Test Pull off and dispose cap of lancet / Clean fingertip with alcohol pad. Press the lancet against the fingertip. Massage the pricked fingertip to obtain blood drop. Put capillary dropper in contact with blood and draw the blood to fill line. Put the collected blood into the sample well. Add 2 drops of the buffer. Wait for the coloured line and read results at 10 minutes compare it to vitamin D Colour card. Full product preparation and usage is included in product box on IFU instructions for use.Usage Warnings Precautions to consider before using 2San Vitamin D Test Information included in product box on IFU instructions for use Warnings